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immunosorbent assay elisa max standard kit  (Revvity)
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Revvity immunosorbent assay elisa max standard kit
CpG increased IL-10 mRNA expression and protein secretion in spleen B cells from WT and TLR9 KO mice. Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CD40L (0.1 μg/ml), CpG (1 μM) +CD40L (0.1 μg/ml) for 48 h. The levels of IL-10 mRNA expression (A) and protein secretion (C) were detected by qRT-PCR and <t>ELISA,</t> respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01). Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CpG-Low (0.1 μM), CpG-Med (1 μM) and CpG-High (10 μM) for 48 hours. The levels of IL-10 mRNA expression (B) and protein secretion (D) from cultured B cells were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01)
Immunosorbent Assay Elisa Max Standard Kit, supplied by Revvity, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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immunosorbent assay elisa max standard kit - by Bioz Stars, 2026-02
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CpG increased IL-10 mRNA expression and protein secretion in spleen B cells from WT and TLR9 KO mice. Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CD40L (0.1 μg/ml), CpG (1 μM) +CD40L (0.1 μg/ml) for 48 h. The levels of IL-10 mRNA expression (A) and protein secretion (C) were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01). Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CpG-Low (0.1 μM), CpG-Med (1 μM) and CpG-High (10 μM) for 48 hours. The levels of IL-10 mRNA expression (B) and protein secretion (D) from cultured B cells were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01)

Journal: Journal of Applied Oral Science

Article Title: Cytidine-phosphate-guanosine oligodeoxynucleotides in combination with CD40 ligand decrease periodontal inflammation and alveolar bone loss in a TLR9-independent manner

doi: 10.1590/1678-7757-2017-0451

Figure Lengend Snippet: CpG increased IL-10 mRNA expression and protein secretion in spleen B cells from WT and TLR9 KO mice. Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CD40L (0.1 μg/ml), CpG (1 μM) +CD40L (0.1 μg/ml) for 48 h. The levels of IL-10 mRNA expression (A) and protein secretion (C) were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01). Purified spleen B cells separated from WT mice and TLR9 KO mice were cultured with CpG-Low (0.1 μM), CpG-Med (1 μM) and CpG-High (10 μM) for 48 hours. The levels of IL-10 mRNA expression (B) and protein secretion (D) from cultured B cells were detected by qRT-PCR and ELISA, respectively (mean±SD, n=4 WT mice or TLR9 KO mice per group, *p<0.05, **p<0.01)

Article Snippet: We used Mouse IL-10 enzyme-linked immunosorbent assay (ELISA) Max Standard kit (BioLegend) to measure the IL-10 secretion levels in the supernatants of cultured B cells.

Techniques: Expressing, Purification, Cell Culture, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay